论文题目 |
Identifying the geographical origin of protected sea cucumbers(Apostichopus japonicus) in China usin |
作者 |
云振宇,孙昭(外),徐海燕(外),孙志宏(外),张瑶,刘贞(外) |
年度 |
2017 |
发表/出版时间 |
2017/4/30 |
发表期刊/会议 |
Food Sci.Biotechnol |
关键词 |
sea cucumber, geographical origin, random amplified polymorphic DNA, genetic diversity |
摘要 |
Dalian sea cucumber, Yantai sea cucumber, and Weihai sea cucumber, which belong to
Apostichopus japonicus, are protected as geographical indications in China based on their high
nutritional values and medical propertys. The 26 samples, including Dalian sea cucumbers (9 samples)
in Liaoning province, Yantai sea cucumbers (9 samples), and Weihai sea cucumbers (8 samples) in
Shandong province, were individually collected from the designated geographical sea areas and the
genetic relationships and DNA polymorphisms were evaluated by random amplified polymorphic DNA
technology and gene segments sequencing. The RAPD dendrogram showed that the genetic diversity
of the three types of sea cucumbers was rich. The neighbor-joining tree shows that the genetic
relationship of the samples from the adjacent sea areas is closer. It demonstrates that the gene
characteristics of sea cucumbers from different sea areas were obvious and the genetic diversity
analysis by RAPD-PCR can be used as a rapid method for geographical discrimination. |