论文题目 |
Auto-microfluidic thin-film chip for genetically modified maize detection |
作者 |
云振宇,彭丽萍(外),霍胜楠(外),吴欣(外),周俊(外),张洪光(外),李文静(外),蔡奇(外),黄广平(外) |
年度 |
2017 |
发表/出版时间 |
2017/3/24 |
发表期刊/会议 |
Food Control |
关键词 |
Thin-film chip,Genetically modified organism,Detection |
摘要 |
As a thin-film chip method, reverse dot blot hybridization (RDBH) has been employed to detect hazardous
substances, but an automatic RDBH instrument with low workload, high accuracy and stability is
still urgently needed. This paper presents our newly-developed auto-microfluidic thin-film chip (AMTC)
method for multiplex screening of genetically modified (GM) maize. With specific DNA probes for
genetically modified (GM) maize being immobilized on a square nylon thin-film, it was placed into a
micro-reaction cell of the AMTC device. Then biotin-labeled PCR products with target DNA fragments for
template amplification were added to the micro-reaction cell using a microfluidic system. When the PCR
products passed the square nylon thin-film, the target DNA fragments were captured by the complementary
action of DNA, where the signal was visualized with streptavidin link-coupled alkaline phosphatase
color development kit. The sensitivity of GM maize detection reached 0.1% quality percentage
and its stability and consistency could satisfy the requirements for practical applications. Performance
advantages of the ATMC are manifold, being embodied in aspects such as easy and straightforward
operation, low costs and less workload. |